|
KMID : 0861020130280040077
|
|
Korea Journal of Herbology
2013 Volume.28 No. 4 p.77 ~ p.81
|
|
|
Effects of Horse oil on the DNCB-induced Contact Hypersensitivity in Balb/c Mice
|
|
이영선:Lee Young-Sun
김보애:Kim Bo-Ae/박찬익:Park Chan-Ik/유왕근:Yoo Wang-Keun/조재위:Cho Jae-We/김미려:Kim Mi-Ryeo
|
|
|
Abstract
|
|
|
Objectives : Horse oil (HO) has been used long time as the folk medicine of many Asian countries such as Korea, Mongol, China, India and Japan. HO has been used for anti-bacterial, anti-inflammatory, and anti-pruritic purposes in skin. However, it is still largely unknown whether HO modulates the skin condition. In this study, we attempted to evaluate the anti-inflammatory effect of HO on the 1 % of 2, 4-dinitro-1-chlorobenzene (DNCB)-induced contact hypersensitivity in Balb/c mice.
Methods : To find the anti-inflammatory effect of HO, contact hypersensitivity, a local inflammatory response of skin, was induced on the back of Balb/c mice by sensitization and repeated application by 1% DNCB and HO treated 2 weeks on the 1% of DNCB-treated Balb/c mice. Excised mice skins were stained with hematoxylin and eosin and serum IgE level was measured by mouse IgE ELISA kit.
Results : In this study, we found that HO reduced erythema by 1% of DNCB treated Balb/c mice. Also, HO recovered histopathological features such as the thickening of epidermis, hyperkeratosis and the infiltration of inflammatory cells in 1% of DNCB treated Balb/c mice. In addition, HO reduced IgE level on the serum obtained from blood of 1% of DNCB-treated Balb/c mice.
Conclusion : Taken together, these results showed that HO could be used as a pharmaceutical material with anti-inflammatory effects by reducing of erythema, IgE level and recovering of histopathological features skin on DNCB-induced contact hypersensitivity in Balb/c mice model.
|
|
|
KEYWORD
|
|
|
Horse oil, DNCB, Contact hypersensitivity, anti-inflammatory, Balb/c mice, IgE
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|